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KMID : 0388220070140030219
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Journal of the Korean Rheumatism Association
2007 Volume.14 No. 3 p.219 ~ p.226
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Characterization of FSChigh Memory B Cells from Patients with Active Systemic Lupus Erythematosus
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Jhung Joo-Yeon
Kim Ho-Youn
Ju Ji-Hyeon
Park Sung-Hwan
Kim Young-Joo
Chang Soog-Hee
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Abstract
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Objective:To determine phenotypic and functional characteristics of memory B cells in patients with systemic lupus erythematosus (SLE).
Methods:The percentage of memory B cell subsets in peripheral blood mononuclear cells (PBMC) from normal control (n=11), inactive (n=15) and active (n=10) SLE patients was determined by Fluorescence Activated Cell Sorter (FACS). In addition, the activation status of memory B cells was measured by the surface expression of CD86 (B7-2). The production of antibodies to chromatin and dsDNA (IgG and IgM type) by isolated memory B cell subsets was examined by enzyme-linked immunosorbent assay (ELISA).
Results:In this study, we analyzed 2 subtypes of memory B cells: FSC (Forward Side Scatter)low and FSChigh memory B cell. The percentage of both subtypes from active and inactive SLE patients was significantly reduced compared to that of normal controls (p＜0.01). In addition, the expression of activation markers, CD86 on FSChigh memory B cells from active SLE patients was higher than those of inactive SLE patients and normal controls (p=0.014). Upon stimulation with CpG and IL-15 in vitro for 8 days, isolated FSChigh memory B cells from active SLE patients revealed augmented production of autoantibodies to chromatin and dsDNA.
Conclusion:Our results suggest that abnormally activated FSChigh memory B cells from active SLE patients might be involved in spontaneous production of autoantibodies and induce transition from inactive to active phase of the patients.
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KEYWORD
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Systemic lupus erythematosus, Anti nuclear autoantibodies, Memory B cell CD86 (B7-2), CPG
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